Journal: Endocrinology

Article Title: ROS1 Signaling Regulates Epithelial Differentiation in the Epididymis

doi: 10.1210/en.2014-1341

Figure Lengend Snippet: Pharmacological inhibition of ROS1 or MEK1/2 in prepubescent mice prevents IS epithelia differentiation. A, Western blot analysis of lysates from caput epididymides from ROS1 wild-type mice treated with either crizotinib or PD325901 for 0, 5, and 12 days against the indicated proteins. B, Photomicrographs of paraffin-embedded sections from caput epididymides of (A) stained with H&E or for expression of phospho-ERK1/2 by IHC. Scale bar, 250 μm.

Article Snippet: Drug treatment Crizotinib and PD325901 (LC Labs) were reconstituted in DMSO and diluted in Ora-Plus (Paddock) or PBS and administered by oral gavage or ip injection.

Techniques: Inhibition, Western Blot, Staining, Expressing

Journal: Endocrinology

Article Title: ROS1 Signaling Regulates Epithelial Differentiation in the Epididymis

doi: 10.1210/en.2014-1341

Figure Lengend Snippet: Peripubertal inhibition of ROS1 kinase activity has no long-term effect on architecture of IS epithelium and fertility. A, Western blot analysis of caput (including IS) from males that were treated with crizotinib between P17 to P28 and aged to P70 for the levels of phospho-ROS1. P21 untreated caput epididymides were used as a positive control. B, The heights of IS epithelium were measured in 2 control and 2 crizotinib-treated (from P17 to P28) in juvenile males immediately posttreatment (P28) and 42 days after cessation of treatment (P70 adult). *, P < .05. C, Peripubertal crizotinib-treated and control mice (N = 5 each) as in (B) were aged to adulthood (10-weeks-old) and mated with 6-week-old females. Copulatory vaginal plugs and pregnancies were recorded and percent fertility calculated as the number of pregnancy/plugs.

Article Snippet: Drug treatment Crizotinib and PD325901 (LC Labs) were reconstituted in DMSO and diluted in Ora-Plus (Paddock) or PBS and administered by oral gavage or ip injection.

Techniques: Inhibition, Activity Assay, Western Blot, Positive Control, Control

Journal: Endocrinology

Article Title: ROS1 Signaling Regulates Epithelial Differentiation in the Epididymis

doi: 10.1210/en.2014-1341

Figure Lengend Snippet: Inhibition of ROS1 and MEK1/2 kinase activity in adults. A, Western blot analysis of lysates from caput epididymides (including IS) from ROS1 wild-type mice treated with either crizotinib or PD325901 for 0, 7, and 14 days against the indicated proteins. B, Photomicrographs of paraffin-embedded sections from proximal epididymides treated as per (A) and stained with H&E or phospho-ERK1/2 by IHC. Scale bar, 250 μm. C, Western blot analysis of lysates from proximal caput epididymides (including IS) from ROS1 wild-type mice treated with crizotinib for 12 days. D, Crizotinib-treated and control mice were mated with 6-week-old females and copulatory vaginal plugs and pregnancies were recorded, and percent fertility calculated from the number of pregnancy/plugs.

Article Snippet: Drug treatment Crizotinib and PD325901 (LC Labs) were reconstituted in DMSO and diluted in Ora-Plus (Paddock) or PBS and administered by oral gavage or ip injection.

Techniques: Inhibition, Activity Assay, Western Blot, Staining, Control